By Barbara J. Bain
- permits either the haematologist and laboratory scientist to spot blood mobilephone beneficial properties, from the most typical to the extra obscure
- presents crucial info on tools of assortment, blood movie guidance and marking, including the rules of guide and automatic blood counts
- thoroughly revised and up-to-date, incorporating a lot newly released info: now contains suggestion on extra exams while a selected prognosis is suspected
- four hundred prime quality pictures to assist with blood phone identification
- Highlights the aim and medical relevance of haematology laboratory assessments all through
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Additional info for Blood Cells A Practical Guide, 5 edition
If necessary, a reticulocyte preparation can be counterstained with a Perls stain to identify Pappenheimer bodies, or by a Romanowsky stain, to identify Howell–Jolly bodies. g. the new methylene blue, is washed out during the methanol ixation. The reticulum is then stained by the basic component of the Romanowsky stain . Reticulocytes are usually counted as a percentage of red blood cells. The use of an eyepiece containing a Miller ocular micrometer disc (Fig. 3 The characteristic appearance of various red cell inclusions on a new methylene blue reticulocyte preparation.
A ixed rigid cell will appear larger than its actual volume. Furthermore, cell deformability is a function of haemoglobin 31 concentration within an individual cell. The effect of cell shape is not the same with all impedance counters. In one study the inaccuracy was greater with a Coulter STKR and a Cell‐Dyn 3000 than with a Sysmex K‐1000 and was not seen with a Sysmex NE‐8000 . Beckman–Coulter (previously Coulter) instru ments initially measured Hb by a modiied cyanmeth aemoglobin method.
G. haemoglobin in luid salvaged during surgery or haemoglobin in plasma or urine . Hb can be measured as oxyhaemoglobin, in which case concentration of carboxyhaemoglobin, sulphaemoglobin and methaemoglobin will not be measured accurately. An artiicial or secondary standard is needed. This method has been incorporated into directly reading haemoglobinometers, which are standardised to give the same result as a cyanmethaemoglobin method. Hb can be measured as haematin produced under alkaline conditions.
Blood Cells A Practical Guide, 5 edition by Barbara J. Bain