Download PDF by Margaret H. Baron: Developmental Hematopoiesis: Methods and Protocols

By Margaret H. Baron

ISBN-10: 1588292967

ISBN-13: 9781588292964

The 1st number of ways to research embryonic and fetal hematopoiesis in either invertebrate (fruit flies) and vertebrate (frog, fish, mouse, chick, and human) organisms. those state of the art ideas diversity from the genetic, molecular, and mobile, to phone and embryo explant tradition and entire animals, together with in vivo imaging. Bioinformatic and sensible genomic methods for learning stem cells and their supportive stromal cells also are mentioned. The protocols stick to the profitable tools in Molecular Medicineв„ў sequence structure, each supplying step by step laboratory directions, an advent outlining the main at the back of the procedure, lists of the mandatory gear and reagents, and pointers on troubleshooting and fending off recognized pitfalls.

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And Tomasiewicz, H. (1998) Using Flp-recombinase to characterize expansion of Wnt1-expressing neural progenitors in the mouse. Dev. Biol. 201, 57–65. 43. , and Berns, A. (1998) Flp-mediated tissuespecific inactivation of the retinoblastoma tumor suppressor gene in the mouse. Oncogene 17, 1–12. Genetic Manipulation in the Mouse 19 44. , and Orkin, S. H. (1999) Improved reporter strain for monitoring Cre recombinase-mediated DNA excisions in mice. Proc. Natl. Acad. Sci. USA 96, 5037–5042. 45. , Orkin, S.

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And Orkin, S. H. (2001) Activation of EGFP expression by Cre-mediated excision in a new ROSA26 reporter mouse strain. Blood 97, 324–326. 76. , Grone, H. , et al. (2001) Characterization of a novel EGFP reporter mouse to monitor Cre recombination as demonstrated by a Tie2 Cre mouse line. Genesis 30, 36–44. Genetic Manipulation in the Mouse 21 77. , Schlaeger, T. , et al. (2001) Endothelium-specific replacement of the connexin43 coding region by a lacZ reporter gene. Genesis 29, 1–13. 78. , and Ikehara, S.

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Developmental Hematopoiesis: Methods and Protocols by Margaret H. Baron

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